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 BioPak® MicroColumn™ QuicKits™ for the Purification of BioMolecules Using Chromatography Resins.
 MicroColumn Chromatography Kits. Invented by NSS. Copied by Others.
Overview
BioPak MicroColumns offer a revolutionary system for isolating and purifying proteins, peptides, ssDNA, oligonucleotides, dsDNA, and other biomolecules using chromatography resins sealed between two porous balls in a pipet tip. Protocols are performed, with a 200ul hand-held pipettor, simply by pipetting the solution up and down through the resin bed. Biomolecules purified in this manner are now ready for protein digestion, cloning, direct sequencing reactions, DNA amplifications, gel electrophoresis, HPLC, mass spectrometry, and other techniques.
General Procedure
Easy-to-use MicroColumn Protocols consist of three rapid steps. First, the molecule of interest is mixed with a binding solution, and applied to the resin by pipetting. Second, the unbound contaminants are removed from the resin with a wash solution. Third, the target molecule is eluted with an appropriate reagent. Each of these steps is done by simply pipetting the solution up and down several times.
Advantages
Disposable, low pressure, bi-directional flow MicroColumns now enable many types of column chromatography to be reduced to a micro-volume level. This technology simplifies the performance of complex purification schemes. Design permits multiple purification steps to be performed with ease in less time than it takes to perform a single purification step using another method. As this process is time dependent, reducing the time the target molecule remains bound to the resin increases the potential for recovering biologically active molecules. And, because MicroColumns are mounted from a rack onto a pipettor, they never need to be handled directly, eliminating contamination.
Resins Packed
MicroColumns, for both manual and automated pipettors, are currently available pre-packed with the following resins: (Click on Green, Rose, or Violet BioPak MicroColumn QuicKit links below to access technical protocols.)
- Green BioPak® MicroColumns™ filled with C-18 resin, for purifying proteins, peptides, ssDNA, and oligonucleotides.
- Rose BioPak® MicroColumns™ filled with DEAE resin, for isolating proteins, purifying PCR products from amplification reactions, cleaning-up DNA from enzymatic reactions, and purifying plasmid DNA from bacterial lysates.
- Violet BioPak® MicroColumns™ filled with GlasPac resin, for purifying DNA from alkaline lysis, tris buffer solutions, agarose gel slices, or other sources.
- Empty MicroColumns™ are also available to researchers who prefer to pack their own resins, allowing for unique purification strategies.
Manual Pipetting Procedure
Universal-fitting, BioPak/M MicroColumns are designed for use with single or multi-channel pipettors such as: Biohit, Brinkmann, Eppendorf, Pipetman, Finnpipette/Labsystems, BioTech, Costar, Socorex, Hamilton, and Titertek. MicroColumns for manual pipettors are loaded into special footprint, 96-hole pipet tip racks which are also designed for use with automated High Throughput Screening equipment.
Automated Pipetting Procedure
BioPak/B MicroColumn Preps are designed for procedures utilizing the Beckman Biomek 1000/2000/SL Automated Workstation. Unique 96-tip VacRak fits the Biomek stage and is engineered so that each tip is isolated in an individual well, eliminating cross-contamination between samples. VacRaks can be pre-loaded with BioPak/B MicroColumns at selected locations and with standard Biomek tips in remaining positions. This allows micro-chromatography to be integrated into a complex pipetting sequence. Excellent results have been achieved with BioPak MicroColumns on a Biomek Workstation, for example, to automate plasmid DNA isolation, and for isolating glyco proteins. Novel design, and the ability to produce MicroColumns filled with a wide range of resins, make them essential tools for laboratories where the isolation and purification of biomolecules is standard procedure. U.S. and Foreign Patents. |
