Glas Matrix DNA/Plasmids Isolation Kits. Invented by NSS. Copied by Others.
MaxiPlas/GlasPac QuicKit addresses two separate methodologies for purifying and concentrating ds/ss DNA ranging from 10 base pairs to 20,000bp.
The first half of the kit utilizes three reagents, combining an RNA digestion and alkaline lysis procedure, for isolating plasmid DNA from bacterial
cell cultures. The second half of the kit utilizes GlasPac, our high-binding glass matrix, and two reagents to purify nucleic acids from TAE or TBE agarose gels or solutions, and to free plasmid DNA
of contaminating proteins, oligo RNA, and enzymes. GlasPac has a binding capacity of 1ug of DNA per 1ul of GlasPac, and can be eluted in a volume as small as 5ul. Recoveries range from 70% to
90% from bacterial cells and agarose gels, and virtually 100% from solutions. Each GlasPac purification provides an excellent substrate for restriction enzyme digestions, ligations, transformations,
sequencing and amplification procedures.
For purifying DNA from gels and solutions only, Click Here to see GlasPac/GS OuicKits.
REAGENTS
#1 Buffer (60ml): Buffer containing highly purified DNase-free, RNase-A.
#2 SDS (60ml): Alkaline Buffer (Sodium Dodecyl Sulfate).
#3 KAC (60ml): Acidic Buffer (Potassium Acetate).
#4 Salt (60ml): Saturated Sodium Iodide Solution.
#5 Wash (60ml): Concentrated Sodium Chloride Solution, yielding 400ml of prepared wash.
GlasPac (2.0ml): DNA Glass-Binding Matrix.
GENERAL INFORMATION & PROTOCOLS
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MaxiPlas/GlasPac General Information Pamphlet
